PyHDX is software to extract H/D exchange kinetics from HDX-MS data sets in terms of Gibbs free energy of exchange (ΔG) at the residue level.

An interactive web server is available where users can upload HDX-MS data and obtain ΔG values. Please refer to the GitHub page for up-to-date links to the web server. How to use the web app is described in PyHDX web application.

PyHDX analysis can also be ran headless from python scripts. The ‘templates’ directory on GitHub lists several examples. Further examples can be found in the section Examples.

For more information, please have a look at our manuscript on bioRxiv.